Table 4.
Growth parameters of the four xenic and one axenic strain of Prorocentrum cordatum.
| 20°C | 26°C | 30°C | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Strain | Growth rate (μd-1) | Doubling time (days) | Day of highest cell density | Growth rate (μd-1) | Doubling time (days) | Day of highest cell density | Growth rate (μd-1) | Doubling time (days) | Day of highest cell density |
| CCMP697 | 0.38 | 1.81 | 25.87 | 0.44 | 1.59 | 10.63 | 0.54 | 1.27 | 15.06 |
| CCMP698 | 0.27 | 2.56 | 28 | 0.48 | 1.44 | 10.63 | NA | NA | NA |
| CCMP1529 | 0.16 | 4.44 | 26 | 0.35 | 1.98 | 14.48 | NA | NA | NA |
| CCMP2811 | 0.36 | 1.92 | 18.10 | 0.50 | 1.37 | 12.83 | 0.42 | 1.64 | 8.92 |
| CCMP1329* | 0.38 | 1.83 | 15.26 | 0.46 | 1.50 | 15.18 | 0.30 | 2.26 | 13.00 |
The cell concentrations (cell/ml) determined by flow cytometry for three independent growth curves at each temperature were fitted to a generalized additive model (GAM). Growth parameters were obtained as described in Materials and methods. Supplementary Figure 4 shows calculation of growth rate.
*
Axenic. NA, strains CCMP1529 and CCMP698 were not able to grow at 30°C.