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Microchannel device designed for the application of acute focal hypoxia in the human cortical brain model. Schematic diagrams for the device at 3D view (A), side view (B) and top view (C). Oxygen is only scavenged from the middle chamber where only axons are allowed to grow into. (D) Positions of the oxygen concentration measured were indicated by crosses in the respective colours. (E) Plot of oxygen concentration at different positions under eLOS, measured by a gold electrode.
