Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 12;8(41):eabo2510. doi: 10.1126/sciadv.abo2510

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

PMC Copyright notice

Fig. 4. — (A) LD staining (BODIPY 493/503) in bnip3^−/− HCC cells reconstituted with EV, HA-BNIP3^WT, or HA-BNIP3^W18A ± 100 μM oleic acid. (B) Graph of BODIPY 493/503 staining in bnip3^−/− HCC cells reconstituted with EV, HA-BNIP3^WT, or HA-BNIP3^W18A (***P < 0.001; ****P < 0.0001; at least 20 cells per field, each data point is one field, n ≥ 10 fields per condition). (C) Graph of BODIPY 493/503 staining in bnip3^−/− HCC cells reconstituted with EV, HA-BNIP3^WT, or HA-BNIP3^W18A + oleic acid (***P < 0.001; ****P < 0.0001; at least 20 cells per field, each data point is one field, n ≥ 10 fields per condition). (D) Quantification of BODIPY 493/503 ImageStream flow cytometric analysis of bnip3^−/− HCC cells reconstituted with EV, HA-BNIP3^WT, or HA-BNIP3^W18A (***P < 0.001 and ****P < 0.0001; at least 10,000 cells per condition were quantified for n = 2 experiments). (E) Quantification of cell size generated by ImageStream flow cytometric analysis of bnip3^−/− HCC cells reconstituted with EV, HA-BNIP3^WT, or HA-BNIP3^W18A (***P < 0.001 and ****P < 0.0001; at least 10,000 cells per condition were quantified for n = 2 experiments). (F) Lipid droplet staining (BODIPY 493/503) in bnip3^−/− HCC cells reconstituted with EV, HA-BNIP3^WT, or HA-BNIP3^W18A grown for 6 hours in 100 μM oleic acid and then released into lipid-free media for 0, 8, and 16 hours. (G) ImageStream flow cytometric quantification of relative numbers of LDs in bnip3^−/− HCC cells reconstituted with EV, HA-BNIP3^WT, or HA-BNIP3^W18A grown for 6 hours in 100 μM oleic acid and then released into lipid free–media for 0, 8, and 16 hours (at least 10,000 cells per condition were quantified for n = 2 experiments).