Figure 3. RTK expression and Ras activity can be rapidly remodeled in response to inhibitor treatment.

(A) Dynamic and inhibitor-specific changes in RTK expression are observed over an acute inhibitor time course and in long-term resistant NCI-H358 cells (R). (B) KRAS activity is rapidly restored when cells are dosed with KRAS^G12C or MEK inhibitors. Long-term exposure to MEK inhibitor (AZD4785, R) also results in up-regulation of HRAS activation. In all cases naïve NCI-H358 cells were treated with vehicle control (−) or IC₉₀ concentrations of each inhibitor for the indicated time periods with cells re-dosed every 48 h. Blots are representative of n = 3 biological repeats.