TABLE 2.

Detailed Metabolic Procedure Specimens Collected in T1DAPC

Specimen Type	Collection	Metabolic Test	Type of Preservative	Short-term Stabilization	Centrifuge	Processing Time	Aliquot	Long-term Storage
Plasma	Blood draw	OGTT (6 time points)	2 ml P800 tube*	4°C (refrigerated or on wet ice)	1200 g at room temperature for 10 min with brake on	<4 h	250 μL × 4 per time point	−80°C
		MMTT (8 time points)	8.5 ml P800 tube*		1200 g at room temperature for 20 min with brake on		250 μL × 4 and 500 μL × 6 per time point
Serum	Blood draw	OGTT (6 time points)	6 ml Silicone-coated (red top)	Room temperature for 30–60 min, then 4°C until centrifugation	1200g at room temperature for 10 minutes with brake on	<4 h	250 μL × 12 per time point	−80°C
		MMTT (8 time points)	4 ml Silicone-coated (red top)				250 μL × 8 per time point
		FSIGTT (21 time points)	3 or 5 ml Silicone-coated (red top)				500 μL × 3 or 500 μL × 5 per time point

^*Mix gently by slowly inverting 8 to 10 times

OGTT indicates oral glucose tolerance test; MMTT, mixed meal tolerance test; FSIGTT, frequently sampled intravenous glucose test.