Geneallele	Primers	Enzyme	wt	mut
mef2dco3013	Two separate reactions are run per sample. First reaction: Fw Full, Rv Full, and Fw wt Second reaction: Fw Full, Rv Full, and Rev mut Fw Full: 5’-AAGAAAGGCTTTAACGGTTGC-3’ Rv Full: 5’-AAGAGAAGGACGGAGGTTAGA-3’ Fw wt: 5’-GACAAATACCGGAAGAGCGA-3’ Rv mut: 5’-AGAGGATATCCAGCTCCTCTTC-3’	None	173 bp and 98 bp	121 bp and 173 bp
mef2aaco3017	Fw: 5’-TTGACCCAACGGTTTACAGA-3’ Rv: 5’-CACAAAGCCAAGCAAAAACA-3’	NlaIII	291 bp and 145 bp	Uncut 442 bp
mef2cb fh288	Fw: 5'-TCCCTGCTTCTCTCTAGGTGACATTTACATCG-3 ‘ Rv: 5'-TCGTGTGGCTCGTTGTACTC-3’	TaqaI	190 bp and 10 bp	Uncut 200 bp
mef2b co3012	Fw: 5’-CGAGATCGCTCTCATCATCTT-3’ Rv: 5’-GACATACTGGAGGTATACAGACCAAA-3’	AciI	112 bp and 38 bp	Uncut 139 bp