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. 2022 Oct 11;11:e77419. doi: 10.7554/eLife.77419

Figure 3. Quantitative comparison of fluorescence intensity and contrast between samples processed by EZ Clear and Fast 3D.

Figure 3.

(A–F) Lightsheet imaging of a mouse brain perfused with Evans blue dye and cleared by EZ Clear (right hemisphere, A–C) and Fast 3D (left hemisphere, D–F) shows that tissue processed with EZ Clear has cleaner signal and less light scattering. (G) Quantitative comparison of mean fluorescence intensity of lectin-649 at different imaging depths (dorsal to ventral axis) shows no significant difference between hemispheres treated with EZ Clear and Fast 3D (n=3, two-way ANOVA and multiple comparisons, error bars represent standard deviation [SD], ns – not significant). However, (H) while the lectin-649 fluorescence signal contrast remains sharp in EZ Clear treated brain hemispheres (n=3), the contrast of the signal gradually decreases along with imaging depth in the Fast 3D processed brains. (I) Contrast of the lectin-649 fluorescence signal also remains sharp in EZ Clear treated eye, heart, kidney, testis, and ovary.

Figure 3—source data 1. Measurements and comparisons of fluorescent intensity and contrast of lectin-649 between EZ Clear and Fast 3D cleared mouse brains and organs.