. 2001 Dec;183(24):7110–7119. doi: 10.1128/JB.183.24.7110-7119.2001

TABLE 2.

Oligonucleotide primers

Primer no.	Sequence (5′-3′)	Position (template)	Use^a
212	`TCGGGGACTATTACTA`	2449–2464^b	LP
274	`AACCTGTGGTTATGTAT`	3683–3667^b	LP
1366	`CACAGATTGTATGGGGATTAGG`	1364–1385^c ^d	PCR, LP
1367	`CATTTATAGAAAGCACAGTAGC`	2128–2107^c ^d	PCR, LP
1369	`GCTACTGTGCTTTCTATAAATG`	2107–2128^c ^d	PCR, RT-PCR, S
1370	`ATGTGTCAAATAATATTCTTGT`	2657–2636^c ^d	PCR, RT
1947	`TTATTAACCATTAATCATCACC`	1073–1052^c	PCR, PE, S
2980	`AATAAGTAAACAGGTAACGTCT`	1657–1679^e	LP
2981	`GCTCCTTGGAAGCTGTCAGTAG`	2344–2322^e	LP
3333	`CTCTTCCAACTGATTTTATAGC`	2412–2395^c	PE, S
3334	`GCCTCATAATCATATAACCTAA`	933–912^c	PE, S
3534	`AGCATTGCAGGATATAGTTTC`	296–276^g	SOE, S
3644	`CAAGATTTTTCATATACT`	659–642^c	PE, S, LP
4392	`AATACCGTGTAAAGATGTAACATGCTCATTAT`	773–750^c ^f	SOE
4393	`AAAGCTGTACATATAACCTAAATATAAACATA`	923–901^c ^f	SOE
4400	`GGTTATATGTACAGCTTTTTTTGTTTAAGGAG`	955–977^c ^f	SOE
4401	`ACATCTTTACACGGTATTTTTATGTCTATTTT`	802–824^c ^f	SOE
4402	`ATAAAAATAGTAGTTATGCTAAAATATAAAC`	534–555^c ^f	SOE, LP
4403	`CATAACTACTATTTTTATATTTATATTTATCC`	504–482^c ^f	SOE
8034	`CACGGTATTTTTATGTCTATTTT`	802–824^c	LP
8133	`AACATAAAATATAGAACT`	906–889^c	LP

S, sequencing; LP, oligonucleotides used for preparing labeled probes; PE, primer extension.

Refers to relevant position within the Tn4451 sequence (6), GenBank accession no. U15027; see also the work of Lyras et al. (26).

Refers to relevant position within the Tet P determinant (44), GenBank accession no. L20800.

See the work of Lyras and Rood (25).

Refers to relevant position within the Erm B determinant of C. perfringens (8), GenBank accession no. U18931.

Oligonucleotide contains 9 bp at the 5′ end which are complementary to the corresponding primer, which was used in deletion of the putative promoter regions.

Refers to relevant position within the catP sequence (48), GenBank accession no. M74769.1.