TABLE 3.
Plasmid | Mutation | Chloramphenicol MIC (μg/ml)
|
|
---|---|---|---|
E. coli (DH5α) | C. perfringens (JIR33) | ||
None | None | NDa | <0.5 |
pJIR418 | Positive control | >200 | 60 |
pPSV | Vector control | 15 | 1.5 |
pJIR1438 | Wild type | 52.5 | 4 |
pJIR1617 | ΔT1 | >200 | 25 |
pJIR1618 | ΔPE2 | 67.5 | 10 |
pJIR1494 | ΔP3 | 22.5 | 1.5 |
pJIR1644 | P3-525ΔA | ND | 2.5 |
pJIR1645 | T1-G941A | ND | 25 |
ND, not done.