FIGURE 5.

Comparison of the replication kinetics of ZIKV in SFFs isolated from WT and IFNAR2 ^−/− sheep. Primary fibroblasts derived from WT and IFNAR2 ^−/− sheep fetuses were infected at a multiplicity of infection of one with each of the following three strains of ZIKV: MR-766, P6-740, and PRVABC-59. At the indicated time points after infection, cell culture supernatants were harvested to determine the yield of progeny virions using a standard plaque assay on Vero cells. The virus titers in plaque-forming units per ml (PFU/ml) are plotted as a line graph of the average with standard deviation of two replicates. Statistical comparisons were done using a two-tailed t-test at each time point for each viral strain produced from WT and IFNAR2 ^−/− fibroblasts. p-values are color-coded: green (MR-766), orange (P6-740), and red (PRVABC-59).