TABLE 1.

PCR primers for the amplification of IFNAR1 and IFNAR2.

Gene/Exon	Sequence (5′–3′)	Amplicon (bp)
IFNAR1/Exon 2	Forward: CTT​CCC​ATG​CTG​AGA​ACA; Reverse: GTCGGAACTCTTAGACCA	491
IFNAR1/Exon 4	Forward: AGT​CGT​TGC​CAC​CCT​TCT; Reverse: TCG​GGA​TAA​ACA​GTT​TCA​GT	529
IFNAR2/Exon 1	Forward: AGC​GTT​TCT​CGT​GAT​GTA; Reverse: TCCACTTGGCTCTTGACC	418
IFNAR2/Exon 1 a	Forward: CCA​TTC​TTT​ACA​CTG​AGG​ATA​C; Reverse: GCT​ATT​GGT​CCT​GCA​AGC​T	1,706
IFNAR2/Exon 3	Forward: TGG​TGC​TGA​GTT​CCT​GTA; Reverse: TGTAGAAATTGGCTTTGG	593
IFNAR2/Exon 4	Forward: ACC​TTT​GAG​CAG​AGC​CAC​A; Reverse: AGAAGCCAAGTAACCACT	442

^aThis primer set was created to detect a large 1,282 bp deletion found in the IFNAR2 ^−/− rams from colony #57.