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. 2022 Oct 12;610(7931):319–326. doi: 10.1038/s41586-022-05277-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a. Representative images of SATB2 and CTIP2 expression in t-hCO. b. Example images of SOX2 and NeuN expression in t-hCO. c. Representative images of rat-edothelial-marker-1 (RECA1) and IBA1 expression in t-hCO and nearby rat cortex 3 months after transplantation. d. HNA and IBA1 expression in t-hCO. Absence of overlap between HNA and IBA1 suggests microglia originate from rat. e. GFAP and IBA1 expression in t-hCO and rat cortex. All images were acquired 7–9 months in differentiation unless otherwise stated.