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. 2022 Oct 12;13:6005. doi: 10.1038/s41467-022-33434-2

Fig. 2. Nopp140 is essential for Cajal body assembly.

Fig. 2

a Western blots of total lysate from three biological replicates of HeLa cells depleted of Nopp140 or coilin using siRNA transfection targeting Nolc1 and coil mRNAs, respectively. Molecular weight markers indicated to the right of the blots. Representative images for cells undergoing transfection with a non-targeting siRNA (b), siCoil oligo pool (c), or siNolc1 oligo pool (d). Scale bars = 10 µm. SMN and Coilin signal imaged under identical conditions and displayed at the same levels. e Violin plots of Cajal body count per nucleus based on automated quantification of coilin immunostaining. N = 140 (non-targeting siRNA); 103 (siCoil); 142 (siNolc1). Violin plot area is normalized between samples, white dots represent the median, box denotes the interquartile range, and the whiskers note 1.5 the interquartile range. Source data are provided as a Source Data file.