Fig. 5. Point mutations alter NTD capacity to form fibrils and puncta.

a Coil^−/− MEFs transfected with constructs for cytoplasmic expression (NTD) or for nuclear expression (NTD^NLS) of the coilin NTD bearing selected point mutations. The NTD was immunostained with anti-myc (magenta) and DNA visualized with DAPI (cyan). Color scale bars given in analog-digital units. Scale bar = 10 µm. Percentage to the lower right indicates the fraction of cells with the displayed phenotype where n = 300 cells. Images acquired with DeltaVision. b Coil^−/− MEFs transfected with wild-type and mutant coilin nuclear expression constructs (NTD^NLS). NTD was immunostained with anti-myc (magenta), counterstained with anti-Nopp140 (cyan), and DNA visualized with Hoechst (gray). The dashed boxes have been magnified in the inset panels. Imaged with Leica SP8 laser scanning confocal microscope. Color bars are in analog-digital units to indicate differences in expression. Scale bar = 10 µm; inset, 1 µm.