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. 2022 Oct 12;12:17084. doi: 10.1038/s41598-022-20945-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Synaptophysin immunostaining in hippocampal tissues of the studied groups. (a) Section of the hippocampal tissue in the control group showed intense cytoplasmic expression of synaptophysin in neural cells. (b) Section of the hippocampal tissue in the D-gal group showed mild cytoplasmic expression of synaptophysin in neural cells. (c) Section of the hippocampal tissue in the D-gal + Met group showed moderate cytoplasmic expression of synaptophysin in neural cells (Synaptophysin × 200 for A, B and C). (d) Represents the percentage of synaptophysin expression in the above-mentioned groups (*significant when compared to control group, ^#significant when compared to D-gal group). Data are shown as means + SD (n = 10). ANOVA was used to make group comparisons; Significance = P < 0.05).