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. 2022 Apr 27;47(12):2140–2149. doi: 10.1038/s41386-022-01327-z

Fig. 2. mRNA quantification in the CeM of prenatally exposed male and female adolescents.

Fig. 2

(A) Quantification of nuclei within this region. Neither sex nor exposure changed the # of nuclei counterstained by DAPI. (B) Quantification of CRFR1 + cells, reported as % of nuclei-stained cells. PAE significantly decreased the # of CRFR1 + cells in the CeM of males, while significantly increasing CRFR1 + cells in females. (C) Quantification of CB + cells, reported as % of nuclei-stained cells. In controls, males exhibited higher proportions of CB + cells than females. PAE significantly decreased the proportion of CB + cells in the CeM in males, without affecting females. (D) Quantification CB + cells co-labeled with CRFR1 mRNA. Factors of sex and exposure do not change the proportion of CRFR1/CB + cells in the CeM. (E) Quantification of SST + cells in the CeM, reported as % of nuclei-stained cells. PAE significantly decreased the proportion of SST + cells in the CeM in males, without affecting females. (F) Quantification SST + cells co-labeled with CRFR1 mRNA. Factors of sex and exposure do not change the proportion of CRFR1/SST + cells in the CeM (G) Representative images (40X) of fluorescently labeled mRNA in CeM cells across sex and exposure. Red: SST. Blue: CB. Yellow: CRFR1. *indicates significant effect of exposure (*p < 0.05), (**p < 0.01), (***p < 0.001).