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. 2022 Apr 27;47(12):2140–2149. doi: 10.1038/s41386-022-01327-z

Fig. 3. Change in mIPSCs following bath application of selective CRFR1 agonist, Stressin-1, in males and females.

Fig. 3

(A, B) CRFR1-regulated changes in mIPSC frequency in adolescent males, reported as % change from baseline activity. CRFR1 activation produces significant concentration-dependent changes in both control and PAE adolescents. In control animals, 100 nM and 1 µM Stressin-1 significantly decreased mIPSC frequency to similar degrees, while PAE animals exhibit a blunted decrease at 100 nM which is recovered at the highest concentration. (C) Bar graph of % change in mIPSC frequency from control and PAE males following bath application of 100 nM Stressin-1, which revealed a significant effect of exposure in a blunted attenuation of mIPSC frequency in PAE males. (D, E) CRFR1-regulated changes in mIPSC frequency in females, reported as % change from baseline activity. 100 nM Stressin-1 significantly attenuated mIPSC frequency in both control and PAE females. However, 1 µM Stressin-1 resulted in a loss of that attenuation in PAE females, while control females continue to show that consistent reduction in mIPSC frequency. (F) Bar graph of % change in mIPSC frequency from control and PAE females following bath application of 1 µM Stressin-1. *indicates significant effect of exposure (p < 0.05) # signifies significant difference from 0.