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. 2022 Apr 27;47(12):2140–2149. doi: 10.1038/s41386-022-01327-z

Fig. 5. Behavior in the LDB between exposures, sexes and infusions of either ACSF or 100 nM Stressin-1 into the CeA.

Fig. 5

(A) Total time (s) spent in the light chamber of the LDB assay. There was a significant drug effect in control males, with Stressin-1 infusion reducing the time spent in the light chamber. In females, there was a significant effect of exposure only in ACSF-infused animals, with PAE reducing time spent in the light chamber. (B) Time spent in the light chamber by control males, across 3 min time bins of the test. Stressin-1 infused males spend significantly less time in the light chamber than ACSF-infused males. (C) Time spent in the light chamber by PAE males, across 3 min time bins of the test. Drug infusion did not change time spent in the light in PAE males. (D) Time spent in the light chamber by females infused with ACSF, across 3 min time bins of the test. PAE females spent significantly less time in the light chamber than control females. (E) Time spent in the light chamber by females infused with Stressin-1, across 3 min time bins of the test. There was no effect of exposure on light time in Stressin-1-infused females. (F) Transitions between light and dark chambers in the LDB. Males demonstrated a significant exposure x drug infusion interaction, revealing a Stressin-1-induced reduction in transitions in control males and a Stressin-1-induced increase in transitions in PAE males. (G) Transitions in the LDB by control males, across 3 min time bins of the test. ACSF-infused males demonstrated a consistent rate of transitions throughout the test, whereas Stressin-1-infused males demonstrate fewer, less consistent transitions. (H) Transitions in the LDB by PAE males, across 3 min time bins of the test. Drug infusion did not change transition patterns in PAE males. (I) Transitions in the LDB in ACSF-infused females, across 3 min time bins of the test. Control females transitioned more throughout the test than PAE females. (J) Transitions in the LDB in Stressin-1-infused females, across 3 min time bins of the test. Exposure did not change transition patterns in response to Stressin-1 infusion. & indicates significant effect of Stressin-1 (p < 0.05), *indicates significant effect of exposure, $ indicates a significant interaction between drug infusion and prenatal exposure.