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. 2022 Aug 22;47(12):2071–2080. doi: 10.1038/s41386-022-01413-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A PCA visualization of male and female RNAseq samples across brain regions and treatment conditions. B Number of DEGs across brain regions and sexes within each sex and brain region. C Violin plots of Cnr1 and Dagla expression changes across sexes and brain regions. D Pathway enrichment for DEGs in female Amy, DMS, and male NAc. Dot color depicts the direction of regulation and dot size illustrates the significance. E Number of DEGs for treatment by sex interaction across brain regions in analyses including both sexes for each brain region.