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Illumination profile at different modes of excitation with single-mode fiber (SMF). A)Epi mode (0–80 DU). B) At a critical angle (0–660 DU). C) At the TIRF mode (0–345 DU). Lines indicate the position used for making the 30-pixels-wide line profiles. D) Horizontal, and E) vertical line profiles. The sample is a glass coverslip covered with sAv-ATTO655 molecules.
