. 2001 Dec;183(24):7198–7205. doi: 10.1128/JB.183.24.7198-7205.2001

TABLE 1.

Bacterial strains and plasmids

Strain or plasmid	Relevant characteristics^a	Description and/or reference
Strains
E. coli
DH5αMCR	RecA⁻ Gn^s	8
S17-1	RecA⁻ Gn^s	IncP RP4 inserted into the chromosome (19)
HB101	RecA⁻ Gn^s Str^r	11

B. thetaiotaomicron
BT5482	Wild type, Gn^r (G2–G7)⁺ starch⁺	Anaerobe Laboratory, Virginia Polytechnic Institute, Blacksburg, Va.
BT4009	Tc^r Em^r Gn^r (G2-G7)⁺ starch⁺	B. thetaiotaomicron mutant with CTn7853 element carrying Tc^r (tetQ) and Em^r (ermG) genes; known as Tc^r Em^r 7853 (3)
BTMAL	Tc^r Em^r Gn^r G2⁺ G3⁻ starch⁻	Tn4351-generated mutant of BTΩsusR (this study)
Ms-1	Em^r Gn^r G2⁺ G3⁺ starch⁻	Tn4351-generated mutant of strain BT5482 (1); displays BTΩsusR phenotype since susR is disrupted
BTΩsusR	Tc^r Gn^r G2⁺ G3⁺ starch⁻	B. thetaiotaomicron mutant with a susR disruption created by a suicide vector pBT-1 containing 185-bp ClaI-BstYI internal susR; known as susR::pBT-1 (5)
BTΩmalR	Em^r Gn^r G2⁺ G3⁺ starch⁺	B. thetaiotaomicron mutant with a malR disruption created by a suicide vector pGERM containing a PCR-generated 0.33-kbp internal fragment of malR (this study)
BTΩsusRΩmalR	Tc^r Em^r Gn^r G2⁺ G3⁻ starch⁻	BTΩsusR with a malR disruption created by a suicide vector pGERM containing a PCR-generated 0.33-kbp internal fragment of malR (this study)
BTΩsusB	Tc^r Gn^r starch⁻	B. thetaiotaomicron mutant with a susB disruption created by a suicide vector pBT-1 containing a PCR-generated 0.61-kbp internal fragment of susB (16)
BTΩsusB1	Em^r Gn^r starch⁻	B. thetaiotaomicron mutant with a susB disruption created by suicide vector pNJR-6 containing a 0.57-kbp internal fragment of susB; known as susB1::pNJR6 (4)
BTΩsusBΩmalR	Tc^r Em^r Gn^r starch⁻	BTΩsusB with a malR disruption created by a suicide vector pGERM containing a PCR-generated 0.33-kbp internal fragment of malR (this study)
BTΩmalRGUS	Em^r Gn^r G2⁺ G3⁺ starch⁺	B. thetaiotaomicron mutant with a GUS fusion created by suicide vector pΩMALRGUS (this study)
Plasmids
pBT-1	Kn^r Tc^r	RSF1010-based suicide vector used to make insertional disruptions in Bacteroides spp. (21)
pGERM	Ap^r Em^r	pUC19-based suicide vector (expressing ermG) used to make insertional disruptions in Bacteroides spp. (18)
pMALR	Ap^r Tc^r Tc^r	PCR-generated 1.2-kbp DNA fragment containing entire malR and its promoter region cloned into pT-COW (this study)
pMALRGUS	Ap^r Em	PCR-generated 0.82-kbp DNA fragment containing malR promoter region cloned into pMJF-2 (this study)
pΩMALRGUS	Ap^r Em^r	pCQW-1 containing a PCR-generated 0.33-kbp internal fragment of malR (this study)
pT-COW	Ap^r Tc^r Tc^r	pVAL-based shuttle vector with tetQ (7)
pMJF-2	Ap^r Em^r	pFD160-based shuttle vector containing a promoterless GUS gene (6)
pCQW-1	Ap^r Em^r	pUC19-based suicide vector containing a promoterless GUS gene (6)

Abbreviations: G2, maltose; G3, maltotriose; G7, maltoheptaose; Tc, tetracycline; Em, erythromycin; Gn, gentamicin; Ap, ampicillin; Kn, kanamycin; Cm, chloramphenicol; Str, streptomycin. Underlined antibiotic resistances are expressed only in E. coli. Other resistances are expressed only in Bacteroides spp.