Figure 2.

miR-149-3p^−/− (KO) mice are more sensitive to DSS-induced colitis

(A) Percentage of death from WT (n = 8) and miR-149-3p^−/− (KO) mice (n = 17), observed for 11 days after administration of 2% DSS (2% DSS for 7 consecutive days followed by drinking water). (B) WT and miR-149-3p^−/− (KO) male mice at 8 weeks of age were weighed on day 0 and given drinking water (control group) or 2% DSS (w/v) ad libitum for 7 days (n = 5). Disease activity index (DAI) of each mouse was calculated every other day. (C) After 7 days of DSS administration, the weight of mice was recorded and weight reduction was calculated. (D–F) The colon of WT and KO was removed and photographed (D), and colon length (E), and colon weight (F) of each mouse were recorded. (G) Representative hematoxylin and eosin staining of colonic tissues from water- and 2% DSS-treated WT and KO mice are shown (magnification, ×200). (H) Relative mRNA levels of proinflammatory genes of colon from WT and KO mice after DSS administration. WT, wild-type mice; KO, miR-149-3p^−/− mice. ∗p < 0.05.