Figure 3.

miR-149-3p^−/− mouse colon tissues display elevated NF-κB activity and reduced AMPK activity

(A) The phosphorylated IκBα (p-IκBα) levels are upregulated and phosphorylated AMPK (p-AMPK) levels are downregulated in young KO colon compared with WT colon. Total IκBα (T-IκBα) and total AMPK (T-AMPK) were used for normalization of protein levels (n = 5). (B) Immunoblot analysis for the phosphorylated IκBα (p-IκBα) and phosphorylated AMPK (p-AMPK) in total protein pools from aged WT and miR-149-3p^−/− mouse (KO) colon. Total IκBα (T-IκBα) and total AMPK (T-AMPK) were used for normalization of protein levels (n = 5). WT, wild-type mice; KO, miR-149-3p^−/− mice. ∗p < 0.05.