Figure 8.

WT mice co-housed with miR-149-3p^−/− mice (CH-WT) endow susceptibility to DSS-induced colitis

(A) Schematic diagram of the experimental process. WT and miR-149-3p^−/− males at 4 weeks were co-housed for 4 weeks. As a control, a single type control group was fed under the same conditions, and then 2% of DSS was given for 7 days (n = 6). (B) Disease activity index (DAI) was calculated every other day. (C) Weight was determined daily. (D) After 7 days of DSS administration, the colon of WT and KO mice was removed and photographed, and colon length (E) and colon weight (F) of each mouse were measured and recorded. (G) Representative hematoxylin and eosin staining of colonic tissues from the different groups (WT (DSS), KO (DSS), CH-WT (DSS), CH-KO (DSS)) are shown (magnification, ×100). WT (DSS), wild-type mice with DSS treatment; KO (DSS), miR-149-3p^−/− mice with DSS treatment; CH-WT (DSS), WT mice co-housed with miR-149-3p^−/− mice after DSS treatment; CH-KO, miR-149-3p^−/− mice co-housed with WT mice after DSS treatment.