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. 2022 Sep 29;12:1006477. doi: 10.3389/fonc.2022.1006477

Figure 3.

Figure 3

B cell depletion leads to increased lung tumorigenesis. (A) Schematic of B cell depletion by αCD20 in smoking carcinogen urethane-induced endogenous lung tumor model. (B) FACS data showing B cell depletion by αCD20 in blood before first dose of urethane (n = 5). (C) FACS data showing B cell depletion by αCD20 in blood, lung, tumor-draining lymph node (TDLN), and spleen at the endpoint (n = 5). (D) Tumor examination showing increased lung tumor number and tumor burden by αCD20-mediated B cell depletion (n = 5). (E) Tumor examination showing elevated both small and large lung tumor numbers by αCD20-mediated B cell depletion (n = 5). (F) Tumor examination showing enlarged individual lung tumor by αCD20-mediated B cell depletion (n = 5). (G) IHC analysis showing decreased tumor cell apoptosis in mice with αCD20-mediated B cell depletion (n = 5). TDLN: mediastinum lymph node. Data shown were representative of two independent experiments with similar results. Scale bar: 1 mm (D) and 20 µm (G). Student’s t test (two tailed, unpaired) was performed (B-G). Data represented means ± SEM (B-G). *p < 0.05; **p<0.01.