REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-DDI2	This study	N/A
anti-DDI2	Abcam	ab197081
anti-NRF1	Cell signaling	8052
anti-NRF2	Cell signaling	12721, RRID: AB_2715528
anti-Tubulin	Adipogen	AG-27B-0005-C100, RRID: AB_2490494
anti-MT	Enzo Life Sciences	ADI-SPA-550-D, RRID: AB_2039383
anti-ubiquitin	Cell signaling	3936, RRID: AB_331292
anti-K48	Abcam	EP8589
anti-PSMB8	Cell signaling	13726, RRID: AB_2798304
anti-PSMB5	Cell signaling	12919, RRID: AB_2798061
Chemicals, peptides, and recombinant proteins
Bortezomib	LC-Laboratories	B-1408
Carfilzomib	LC-Laboratories	C-3022
H2O2	Sigma-Aldrich	216763-100ML
CdCl2	Sigma-Aldrich	202908-10G
NaAsO2	Sigma-Aldrich	35000-1L-R
K2Cr2O7	Sigma-Aldrich	207802-100g
ZnSO4	Sigma-Aldrich	83265
tBHQ	Sigma-Aldrich	112941-5g
NaF	Sigma-Aldrich	201154
Na4P2O7	Sigma-Aldrich	P8010
MG132	Sigma-Aldrich	c2211-5mg
Suc-LLVY-AMC	Enzo Life Sciences	BML-P802-0005
Z-ARR-AMC	Calbiochem	CAS 90468-18-1
Z-LLE-AMC	Adipogen	CAS 348086-666-8
AMC	Biovision	K245-100-4
Purified human proteasome complexes	Bio-Techne	E−365-025
Critical commercial assays
RNeasy mini kit	QIAGEN	74104
2X Reverse Transcription master mix	Applied Biosystems	4368814
KAPA HiFi HotStart PCR Kit	KapaBiosystems	KK2502
Pierce BCA Protein assay	Thermo Fisher Scientific	23227
TBARS kit	Abnova	KA4409
Catalase activity	Biovision	K773
SOD activity	Biovision	K335
MTS assay	Promega	G1111
Dual-Luciferase Assay System	Promega	E1910
Deposited data
RNA-seq data	Gene Expression Omnibus	GSE198150
Raw data for immunoblots	Mendeley	https://doi.org/10.17632/9krjdj44p5.1
Experimental models: Cell lines
HepG2	Pascal Schneider, University of Lausanne	N/A
Experimental models: Organisms/strains
DDI2flox/flox	This study	N/A
DDI2flox/floxAlbCre+	This study	N/A
DDI2KO	This study	N/A
NRF2lacZ/lacZ	Prof. M. Yamamoto Tohoku University Graduate School of Medicine, Japan	N/A
DDI2flox/floxAlbCre−; NRF2lacZ/lacZ	This study	N/A
Oligonucleotides
RT-PCR ACTIN: fwd 5′-TACCACCA TGTACCCAGGCA-3′ rev 5′-CTCAGGA GGAGCAATGATCTTGAT-3′	This study	N/A
RT-PCR GAPDH: fwd 5′-CGCTC TCTGCTCCTCCTG-3′, rev 5′-CGAT GGTGTCTGAGCGAT-3′	This study	N/A
RT-PCR Mt1: fwd 5′-ATGGAC CCCAACTGCTCCT-3′, rev 5′-AC AGCCCTGGGCACATTT-3′	This study	N/A
RT-PCR Mt2: fwd 5′-CCGATCTC TCGTCGATCTTCAACC-3′, rev 5′-CA GGAGCAGCAGCTTTTCTTGCAG-3′	This study	N/A
RT-PCR NFE2L1: fwd 5′-TGGAACAGC AGTGGCAAGATCTCA-3′, rev 5′-GGCA CTGTACAGGATTTCACTTGC-3′	This study	N/A
RT-PCR NFE2L2: fwd 5′-TTCCCGG TCACATCGAGAG-3′, rev 5′-TCC TGTTGCATACCGTCTAAATC-3′	This study	N/A
RT-PCR MT2A: fwd 5′-CTCTTC AGCTCGCCATGGAT-3′, rev 5′-TG GAAGTCGCGTTCTTTACA-3′	This study	N/A
RT-PCR MT1G: fwd 5′-TTGCAA TGGACCCCAACT-3′, rev 5′-TC CTGGATTTTACGGGTCAC-3′	This study	N/A
RT-PCR NQO1: fwd 5′-CAGCT CACCGAGAGCCTAGT-3′, rev 5′-AG TGCTCTTCTGCCGACCAT-3′	This study	N/A
RT-PCR PSMA4 (human): fwd 5′- CCC TTTGGTGTTTTGCT-3′, rev 5’ – GCT GCAGCGCTATTATTTCC-3′	This study	N/A
RT-PCR Psma4 (mouse): fwd 5’ – AAAAG TGGAAATCGCCACAC-3′, rev 5’ – TTT CTTTCTTCTCCCGCTCA-3′	This study	N/A
SgLuci fwd 5′-CACCGCTTCGAAA TGTCCGTTCGGT-3′, rev 5′- AAAC ACCGAACGGACATTTCGAAGC-3′	(Op et al., 2022)	N/A
SgDDI2 fwd 5′- CACCGGCTCGAAG TCGGCGTCGAC-3′, rev 5′- AAACG GTCGACGCCGACTTCGAGCC-3′	(Op et al., 2022)	N/A
SgNRF1 fwd 5′- CACCGCTTTCT CGCACCCCGTTGTC-3′, rev 5′- AAA CGACAACGGGGTGCGAGAAAGC-3′	(Op et al., 2022)	N/A
Recombinant DNA
LentiCRISPR-v2 vector	Addgene	52961
MT-Firefly-luciferase reporter	Prof. M. Yamamoto Tohoku University Graduate School of Medicine, Japan	(Ohtsuji et al., 2008)
Software and Algorithms
R bioconductor package “limma”	Bioconductor	R version 3.1.1, limma version 3.20.8