Table 2.
Comparison of growth area for the fungi grown in PDA media at increasing concentrations of paclitaxel.
|
H. annosum TC32-1 |
B. adusta strain PAR4 |
Diaporthe
sp. strain PAR5 |
Annulohypoxylon
sp. strain MUS1 |
B. adusta strain PAR7 |
A. arborescens strain PAR15 |
|
|---|---|---|---|---|---|---|
| 0 μM paclitaxel | ||||||
| Day 1 | 12.8 ± 0.8 | 15.2 ± 0.9 | 18.4 ± 1.3 | 20.4 ± 1.4 | 21.2 ± 3.6 | 67.8 ± 2.6 |
| Day 2 | 38.8 ± 2.7 | 147.9 ± 37.4 | 237.9 ± 12.4 | 86.4 ± 10.7 | 361.6 ± 46.3 | 238.9 ± 6.8 |
| Day 3 | 139.1 ± 4.2 | 810.4 ± 160.0 | 968.5 ± 35.6 | 428.8 ± 41.7 | 1,006.1 ± 61.5 | 507.5 ± 8.7 |
| Day 4 | 430.6 ± 58.1 | 1,840.1 ± 189.4 | 2,178.3 ± 52.2 | 931.9 ± 40.5 | 1,897.7 ± 33.4 | 815.6 ± 3.0 |
| Day 5 | 1,019.7 ± 74.2 | 2,544.9 ± 165.6 | 2,614.1 ± 113.1 | 1,272.3 ± 71.3 | 2,563.4 ± 30.5 | 1,112.5 ± 16.8 |
| 23 μM paclitaxel | ||||||
| Day 1 | 13.5 ± 0.2 | 14.0 ± 0.2 | 23.3 ± 1.4 | 19.7 ± 1.7 | 30.9 ± 5.5 | 72.4 ± 0.9 |
| Day 2 | 43.9 ± 1.1 | 90.1 ± 7.6 | 250.7 ± 9.5 | 102.9 ± 14.9 | 400.4 ± 72.9 | 253.2 ± 1.9 |
| Day 3 | 133.2 ± 7.9 | 431.4 ± 1.4 | 877.4 ± 36.4 | 474.3 ± 66.3 | 1,167.4 ± 95.9 | 502.9 ± 2.5 |
| Day 4 | 359.2 ± 70.0 | 1,205.5 ± 93.8 | 1,912.6 ± 78.8 | 988.3 ± 68.3 | 2,181.3 ± 112.6 | 828.4 ± 2.3 |
| Day 5 | 772.4 ± 24.4 | 1,992.8 ± 79.7 | 2,530.5 ± 11.8 | 1,364.3 ± 82.9 | 2,725.2 ± 121.8 | 1,083.8 ± 17.6 |
| 47 μM paclitaxel | ||||||
| Day 1 | 12.9 ± 0.2 | 14.2 ± 0.4 | 22.2 ± 0.6 | 19.0 ± 1.0 | 19.4 ± 2.4 | 65.4 ± 1.7 |
| Day 2 | 34.1 ± 6.0 | 113.9 ± 14.8 | 230.6 ± 12.2 | 78.4 ± 7.7 | 294.4 ± 21.2 | 224.6 ± 2.2 |
| Day 3 | 109.7 ± 9.4 | 526.5 ± 94.6 | 764.2 ± 32.6 | 360.2 ± 24.2 | 875.3 ± 29.3 | 430.4 ± 0.9 |
| Day 4 | 247.8 ± 47.5 | 1,349.7 ± 178.9 | 1,641.8 ± 89.3 | 829.7 ± 28.9 | 1,807.0 ± 54.3 | 704.6 ± 12.8 |
| Day 5 | 615.4 ± 79.4 | 2,223.1 ± 149.2 | 2,279.5 ± 149.2 | 1,220.0 ± 8.0 | 2,563.3 ± 72.9 | 1,013.3 ± 19.3 |
| 70 μM paclitaxel | ||||||
| Day 1 | 14.3 ± 0.3 | 15.6 ± 0.4 | 20.8 ± 0.5 | 18.8 ± 1.9 | 22.9 ± 4.4 | 63.6 ± 0.7 |
| Day 2 | 28.3 ± 2.9 | 109.6 ± 21.0 | 211.3 ± 17.4 | 85.4 ± 18.3 | 310.4 ± 71.1 | 220.1 ± 0.1 |
| Day 3 | 123.7 ± 3.2 | 566.3 ± 83.4 | 733.4 ± 37.4 | 406.8 ± 76.6 | 897.0 ± 171.8 | 419.8 ± 6.4 |
| Day 4 | 324.2 ± 77.2 | 1,501.2 ± 212.8 | 1,621.9 ± 87.5 | 819.4 ± 80.4 | 1,907.8 ± 212.0 | 665.7 ± 20.7 |
| Day 5 | 715.6 ± 91.4 | 2,333.0 ± 231.1 | 2,334.2 ± 137.8 | 1,165.6 ± 94.3 | 2,613.9 ± 189.3 | 953.7 ± 29.0 |
| 94 μM paclitaxel | ||||||
| Day 1 | 11.9 ± 0.9 | 13.6 ± 0.3 | 18.6 ± 0.8 | 17.8 ± 0.3 | 17.5 ± 0.4 | 47.4 ± 0.9 |
| Day 2 | 22.3 ± 4.4 | 114.2 ± 33.9 | 223.0 ± 7.2 | 78.1 ± 5.7 | 279.8 ± 34.9 | 220.4 ± 12.3 |
| Day 3 | 99.4 ± 15.1 | 607.0 ± 89.0 | 745.6 ± 22.0 | 349.2 ± 29.3 | 837.2 ± 40.9 | 425.0 ± 15.9 |
| Day 4 | 215.6 ± 18.7 | 1,601.1 ± 66.1 | 1,652.3 ± 41.3 | 763.8 ± 50.5 | 1,929.1 ± 5.3 | 708.0 ± 20.6 |
| Day 5 | 618.1 ± 33.4 | 2,612.8 ± 97.9 | 2,199.6 ± 61.9 | 1,197.8 ± 44.0 | 2,795.2 ± 29.2 | 988.7 ± 24.2 |
Values are means (mm2) ± standard error. Day 0 was considered the day the fungal plug (5-mm diameter) was placed mycelium-faced to the culture media. DMSO was added to the 0 μM paclitaxel plates. Days 2–5 were considered the exponential growth phase and used in the analysis (Meletiadis et al., 2001; Montini et al., 2006).