Table 3.
Comparison of growth area for the fungi grown in MMN media at increasing concentrations of paclitaxel.
|
H. annosum TC32-1 |
B. adusta strain PAR4 |
Diaporthe
sp. strain PAR5 |
Annulohypoxylon sp. strain MUS1 |
B. adusta strain PAR7 |
A. arborescens strain PAR15 |
|
|---|---|---|---|---|---|---|
| 0 μM paclitaxel | ||||||
| Day 2 | 43.9 ± 6.2 | 434.8 ± 2.2 | 406.8 ± 6.9 | 134.6 ± 7.5 | 280.3 ± 37.8 | 276.1 ± 4.4 |
| Day 3 | 375.5 ± 21.2 | 1,598.2 ± 10.8 | 1,017.9 ± 16.9 | 497.8 ± 10.8 | 1,072.7 ± 110.1 | 559.7 ± 7.1 |
| Day 4 | 1,101.3 ± 46.7 | 2,722.5 ± 33.4 | 1,662.8 ± 25.4 | 928.8 ± 41.5 | 2,169.6 ± 166.2 | 931.9 ± 22.6 |
| Day 5 | 1,539.8 ± 45.1 | 3,145.4 ± 3.3 | 1,948.0 ± 54.8 | 1,319.7 ± 55.8 | 2,706.7 ± 220.2 | 1,292.3 ± 36.5 |
| 23 μM paclitaxel | ||||||
| Day 2 | 47.8 ± 1.2 | 347.7 ± 27.9 | 343.7 ± 11.7 | 124.8 ± 3.1 | 355.3 ± 16.7 | 248.8 ± 4.6 |
| Day 3 | 272.5 ± 5.2 | 1,242.7 ± 52.0 | 882.1 ± 33.1 | 457.2 ± 16.6 | 1,198.7 ± 1.6 | 515.5 ± 5.3 |
| Day 4 | 831.4 ± 15.0 | 2,466.2 ± 104.7 | 1,470.4 ± 43.9 | 943.2 ± 25.0 | 2,221.6 ± 68.7 | 886.2 ± 5.3 |
| Day 5 | 1,366.9 ± 48.4 | 3,062.5 ± 125.6 | 1,779.7 ± 6.8 | 1,414.1 ± 47.1 | 2,801.7 ± 84.9 | 1,240.9 ± 11.4 |
| 47 μM paclitaxel | ||||||
| Day 2 | 44.5 ± 5.6 | 381.5 ± 27.7 | 317.2 ± 3.4 | 108.3 ± 8.2 | 353.2 ± 20.6 | 232.0 ± 4.4 |
| Day 3 | 234.4 ± 2.2 | 1,325.2 ± 73.4 | 784.4 ± 19.8 | 434.9 ± 28.5 | 1,159.7 ± 23.2 | 470.4 ± 1.5 |
| Day 4 | 729.5 ± 21.4 | 2,457.0 ± 43.7 | 1,348.3 ± 15.0 | 844.7 ± 23.1 | 2,267.3 ± 65.7 | 789.9 ± 10.8 |
| Day 5 | 1,263.0 ± 29.7 | 3,093.9 ± 86.4 | 1,650.8 ± 18.0 | 1,362.8 ± 66.2 | 2,793.6 ± 26.6 | 1,121.3 ± 15.7 |
| 70 μM paclitaxel | ||||||
| Day 2 | 40.2 ± 1.9 | 281.1 ± 10.7 | 320.5 ± 4.0 | 129.3 ± 9.6 | 339.6 ± 20.2 | 226.2 ± 5.4 |
| Day 3 | 249.6 ± 14.5 | 1,138.8 ± 18.0 | 793.4 ± 6.4 | 448.9 ± 15.6 | 1,211.1 ± 65.1 | 470.3 ± 12.4 |
| Day 4 | 821.8 ± 43.5 | 2,390.5 ± 6.4 | 1,376.6 ± 21.5 | 903.9 ± 34.0 | 2,275.2 ± 39.6 | 777.4 ± 18.5 |
| Day 5 | 1,430.2 ± 22.7 | 3,006.5 ± 34.4 | 1,716.2 ± 21.5 | 1,318.6 ± 29.2 | 2,834.3 ± 90.8 | 1,084.3 ± 37.4 |
| 94 μM paclitaxel | ||||||
| Day 2 | 43.4 ± 2.4 | 281.8 ± 12.7 | 316.3 ± 2.6 | 121.3 ± 7.9 | 189.0 ± 45.1 | 234.2 ± 3.3 |
| Day 3 | 251.1 ± 11.1 | 1148.6 ± 13.1 | 793.4 ± 16.8 | 418.5 ± 13.5 | 788.9 ± 140.3 | 480.8 ± 5.4 |
| Day 4 | 744.3 ± 40.9 | 2,336.3 ± 72.9 | 1,408.1 ± 17.2 | 864.8 ± 31.3 | 1,668.5 ± 198.2 | 830.3 ± 14.1 |
| Day 5 | 1,371.6 ± 61.3 | 2,942.2 ± 82.8 | 1,727.0 ± 11.0 | 1,382.3 ± 21.9 | 2,252.4 ± 187.8 | 1,194.0 ± 23.6 |
Values are means (mm2) ± standard error. Day 0 was considered the day the fungal plug (5-mm diameter) was placed mycelium-faced to the culture media. Values for Day 1 are missing. DMSO was added to the 0 μM paclitaxel plates. Days 2–5 were considered the exponential growth phase and used in the analysis (Meletiadis et al., 2001; Montini et al., 2006).