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. 2022 Sep 29;12:950043. doi: 10.3389/fonc.2022.950043

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Copyright © 2022 Bai, Shi, Wang, Pan and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of PLAG1 in lung cancer tissue and the relationship between let-7 and PLAG1 as demonstrated by Western blotting and luciferase assays in cells. (A) The expression of PLAG1 in lung cancer tissue was higher. (B–D) The relative PLAG1 expression after transfection of let-7 inhibitor or mimics in lung cancer cell lines. (E) The binding sites of let-7 and PLAG1 and the relative luciferase activity of the let-7 mimic cotransfected with WT/MT were evaluated (ns, no significance; **p < 0.01; ***p < 0.001)..