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The expression of K-ras in cells and the relationship to miR-21 and let-7. (A–C) qRT-PCR and Western blot analyses showing downregulation of K-ras following transfection with K-ras siRNA into H460 and A549 cells. (D, E) qRT-PCR results showing that downregulation of K-ras in H460 and A549 cells resulted in a decrease in the expression of miR-21 and an increase in let-7 compared with the corresponding normal control group (**p < 0.01; ***p < 0.001).
