Figure 3.

Upregulated miR-335-5p in Ang II-induced cardiomyocyte hypertrophy. (a) Representative immunofluorescence micrographs of H9C2 cells stained with ɑ-SMA antibody; scale bars indicate 20 μm; (b) quantitation of average cell surface area; and (c-e) ANP, β-MHC, and miR-335-5p fold changes in cardiomyocytes treated with control, Ang II, and Ang II + miR-335-5p inhibitor. Ang II: angiotensin II. Data are presented as mean ± SD; n = 4 per group; ^∗P < 0.05 and ^∗∗P < 0.01 versus the control group; ^#P < 0.05 and ^##P < 0.01 versus the Ang II group.