Figure 5.

Suppression of miR-335-5p decreased apoptosis and Ca²⁺ accumulation in H9C2 cardiomyocytes induced by Ang II. (a) The apoptosis and intracellular Ca²⁺ accumulation of H9C2 cardiomyocytes were evaluated by TUNEL assay and fluo-3/AM determination; scale bars indicate 50 μm; (b, c) quantified data showing the apoptotic rate and the intensity of Ca²⁺ fluorescence after being transfected with Ang II or miR-335-5p inhibitor (n = 5 per group). Data are presented as mean ± SD; ^∗P < 0.05 and ^∗∗P < 0.01 versus the control group; ^#P < 0.05 and ^##P < 0.01 versus the Ang II group.