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. 2022 Oct 11;10(10):e004653. doi: 10.1136/jitc-2022-004653

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© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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MCs induce apoptosis in murine CRC cells. (A) Tumor-primed MCs were isolated 7 days after KitB6 mice received a single i.p. injection of murine CRC cells (MC38). Once purified, tumor-primed MCs were co cultured with MC38 cells for 2 (B–D) or 5 days (E, F). (B) Gene expression analysis by qPCR for Mki67 (*, p<0.05). (C, D) Representative dot plots of flow cytometric analysis for gating CD45(-) cells or MC38. It was followed by viability and apoptosis analysis. Graph shows percentages of negative or positive MC38 cells for Anexin V (*, p<0.05). (E, F) Representative images of clonogenic assays. Graph shows the relative number of colonies for each group (****, p<0.001). Dots represent individual values together with mean±SEM. P values were calculated using two-tailed Mann-Whitney’s and two-way ANOVA tests (Bonferroni’s post hoc) tests. ANOVA, analysis of variance; CRC, colorectal cancer; MCs, mast cells; ns, not significant.