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. 2022 Oct 11;10(10):e004653. doi: 10.1136/jitc-2022-004653

Figure 2.

Figure 2

MCs induce apoptosis in murine CRC cells. (A) Tumor-primed MCs were isolated 7 days after KitB6 mice received a single i.p. injection of murine CRC cells (MC38). Once purified, tumor-primed MCs were co cultured with MC38 cells for 2 (B–D) or 5 days (E, F). (B) Gene expression analysis by qPCR for Mki67 (*, p<0.05). (C, D) Representative dot plots of flow cytometric analysis for gating CD45(-) cells or MC38. It was followed by viability and apoptosis analysis. Graph shows percentages of negative or positive MC38 cells for Anexin V (*, p<0.05). (E, F) Representative images of clonogenic assays. Graph shows the relative number of colonies for each group (****, p<0.001). Dots represent individual values together with mean±SEM. P values were calculated using two-tailed Mann-Whitney’s and two-way ANOVA tests (Bonferroni’s post hoc) tests. ANOVA, analysis of variance; CRC, colorectal cancer; MCs, mast cells; ns, not significant.