Profiles of ATP- and PPi-dependent PFK
activities in cell extracts of A. methanolica grown in
carbon- and energy source-limited continuous culture on the substrates
glucose, formaldehyde, and methanol at a growth rate of
D (dilution rate) = 0.05 h−1. Days 0
to 6, 50 mM methanol; days 6 to 10, 50 mM methanol plus 5 mM
formaldehyde; days 10 to 14, 50 mM methanol plus 12 mM formaldehyde;
days 14 to 22, 50 mM formaldehyde; day 22 to end, 5 mM glucose. ●,
ATP-PFK; ○, PPi-PFK.