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Profiles of ATP- and PPi-dependent PFK activities in cell extracts of A. methanolica grown in carbon- and energy source-limited continuous culture on the substrates glucose, formaldehyde, and methanol at a growth rate of D (dilution rate) = 0.05 h−1. Days 0 to 6, 50 mM methanol; days 6 to 10, 50 mM methanol plus 5 mM formaldehyde; days 10 to 14, 50 mM methanol plus 12 mM formaldehyde; days 14 to 22, 50 mM formaldehyde; day 22 to end, 5 mM glucose. ●, ATP-PFK; ○, PPi-PFK.
