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Kinetics of ATP-PFK from A. methanolica and effect of PPi on ATP-PFK activity. (A) ATP-PFK activity versus ATP concentration at a fixed F-6-P concentration (10 mM). ▪, 0 mM PPi; ●, 1 mM PPi; ▾, 2 mM PPi; ▴, 4 mM PPi. (B) ATP-PFK activity versus F-6-P concentration at fixed ATP concentrations. ○, 0 mM PPi; ▵, 2 mM PPi; □, 4 mM PPi. Kinetic parameters are given in Table 4.
