Clustal W (36) multiple alignment of the
deduced amino acid sequence of the ATP-PFK from A.
methanolica with the sequences of the PFKs from S.
coelicolor A3(2) (National Center for Biotechnology Information
[NCBI] accession number 008333), E. coli (NCBI
accession number KIECFA), T. pallidum (NCBI accession
number A71366), and T. brucei (NCBI accession number
AAC47836) and the PPi-PFK from A. methanolica (NCBI accession number
Q59126). The percent similarity between the ATP-PFK from A.
methanolica and each of the other PFKs is indicated in
parentheses. A and F indicate residues (solid boxes) involved in
binding of ATP and F-6-P, respectively, in the E. coli
ATP-PFK enzyme (34). *, position with a fully conserved
amino acid residue; :, position with a fully conserved strong group
(STA, NEQK, NHQK, NDEQ, QHRK, MILV, MILF, HY, and FYW); ., position
with a fully conserved weaker group (CSA, ATV, SAG, STNK, STPA, SGND,
SNDEQK, NDEQHK, NEQHRK, FVLIM, and HFY).