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. 2022 Oct 7;9(5):ENEURO.0218-22.2022. doi: 10.1523/ENEURO.0218-22.2022

Figure 1.

Figure 1.

Reduced Gabrg2L inclusion in Mbnl2 KO prefrontal cortex. A, Schematic of custom TaqMan primers for Gabrg2S (Exon 8-Exon 10), Gabrg2L (Exon 8-Exon 9; Exon 9-Exon 10), and primers (Exon 8-Exon 10) for RNA fragment analysis. B, Constitutively expressed Exon 2-Exon 3 of Gabrg2 is not significantly different between genotypes (t(0.99) = 15.33, p = 0.36, Welch’s t test). C, Significant reduction of γ2L inclusion in Mbnl2 KO mice detected via RNA fragment analysis (t(29.43) = 23.11, p < 0.0001, t(14.70) = 14.38, p < 0.0001, Welch’s t test). D, Significant reduction of γ2L inclusion in Mbnl2 KO detected via Taqman assay (t(14.70) = 14.38, p < 0.0001, Welch’s t test). Error bars are ± SEM. E, Strong, positive correlation between PSI values generated from RNA fragment analysis and custom TaqMan assay (r(26) = 0.947, p ≤ 0.0001, Spearman’s correlation). Comprehensive mRNA analyses of Gabaa-R subunits in prefrontal cortex of Mbnl2 KO mice revealed no other subunit difference (Extended Data Fig. 1-1). Full statistical analyses of both experiments can be viewed in Extended Data Figure 1-2.