TABLE 1.
GSI and GSII activities in B. japonicum LP 3001 grown in GN0, GN0.1, and GN1 for 5 or 14 daysa
| Culture age (days) | Culture medium | GS sp act
|
Avg no. of adenylylated GSI subunits | |||
|---|---|---|---|---|---|---|
| Mean nmol of γ-glutamyl hydroxamate min−1 (mg of protein)−1 ± SD
|
% of total
|
|||||
| GSI | GSII | GSI | GSII | |||
| 5 | GN0 | 646.62 ± 33.76 | 1,161.66 ± 60.64 | 35.7 | 64.3 | 1.9 |
| GN0.1 | 275.69 ± 6.73 | 78.00 ± 1.90 | 77.9 | 22.1 | 1.0 | |
| GN1 | 254.60 ± 12.32 | 54.68 ± 2.65 | 82.5 | 17.5 | 1.3 | |
| 14 | GN0 | 1,172.93 ± 89.31 | 278.20 ± 21.2 | 80.8 | 19.2 | 2.3 |
| GN0.1 | 2,153.82 ± 124.97 | 140.98 ± 8.18 | 93.8 | 6.2 | 3.3 | |
| GN1 | 785.76 ± 64.57 | 77.04 ± 6.33 | 91.1 | 8.9 | 6.7 | |
a
The GS activities were measured as γ-GT specific activities and are also presented as percent of total activity. The average number of adenylylated subunits in GSI was deduced by incubations in the presence and absence of 60 mM Mg2+ (46). All determinations were done in quadruplicate.