Table 1.
Esterified sterol fractions in seeds and leaves of wt, slasat1, slpsat1 and slpsat1 x slasat1 mutants.
| Esterified sterol fraction (%) | |||||||
|---|---|---|---|---|---|---|---|
| SEEDS | WT | slasat34 | slasat35 | slpsat28 | slpsat31 | slpsat31 x slasat34 | |
| Total sterols | 37.4 | 60.5 | 60.2 | 5.3 | 4.1 | 3.0 | |
| Cholesterol | 76.3 | 89.9 | 87.0 | 11.1 | n.d. | 16.7 | |
| Campesterol | 40.3 | 62.8 | 46.9 | n.d. | n.d. | n.d. | |
| Stigmasterol | 13.9 | 38.4 | 26.7 | 0.3 | n.d. | 2.7 | |
| Sitosterol | 28.0 | 45.5 | 53.2 | 4.3 | 5.1 | 2.2 | |
| Isofucosterol | 50.3 | 77.3 | 75.2 | n.d. | n.d. | n.d. | |
| LEAVES | WT | slasat34 | slasat35 | slpsat28 | slpsat31 | slpsat31 x slasat34 | |
| Total sterols | 41.2 | 61.5 | 68.9 | 11.6 | 12.0 | 5.2 | |
| Cholesterol | 39.7 | 65.8 | 64.8 | 16.5 | 12.0 | n.d. | |
| Campesterol | 51.4 | 52.1 | 51.8 | n.d. | n.d. | n.d. | |
| Stigmasterol | 7.4 | 26.7 | 23.1 | n.d. | n.d. | n.d. | |
| Sitosterol | 35.5 | 59.8 | 64.8 | 7.8 | 10.1 | 17.3 | |
Values were calculated according to 100*SE/(SE+FS). n.d. stands for not detected. Values shown in bold correspond to the esterified fraction of the total sterols.