FIG. 5.

groESL is under negative control of CtsR in vivo. Expression of groE′-bgaB (QB8133) in the presence (○, ●) or absence (□, ▪) of xylose. Cultures were grown in LB medium at 37°C to an OD₆₀₀ of 0.3 and divided in two, and xylose was added to one of the cultures at a final concentration of 20 mM. Open symbols indicate the OD₆₀₀, and solid symbols indicate β-galactosidase specific activity, expressed as nanomoles of ONP per minute per milligram of protein.