FIG. 6.

CtsR binds specifically to the groESL promoter region. In gel mobility shift experiments (A), radiolabeled DNA fragments (10,000 cpm) were incubated with increasing amounts of purified CtsR. Lanes 1 to 4, 0, 20, 40, and 80 ng of CtsR, respectively. In DNase I footprinting experiments (B), 50,000 cpm of the radiolabeled DNA fragment corresponding to the promoter region was incubated with increasing amounts of purified CtsR. Lanes: 1 to 5, 0, 100, 200, 400, and 800 ng of CtsR, respectively; 6, G+A Maxam and Gilbert reaction of the corresponding DNA fragment. The region protected by CtsR is indicated by a bracket.