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(A) DOC-triggered autolysis is not affected in the ΔclpC mutant. Strain SP2001 (ΔclpC::aphA3) (●) and parental strain R348 (▪) were grown in BHI medium until mid-exponential phase before induction of autolysis by addition of DOC (0.05% final concentration). (B) Growth of the S. pneumoniae R348 wild-type strain and ΔclpC (SP2001), ΔclpP (SP2000), ΔctsR (SP2003), and ΔclpE (SP2002) mutant strains at 37°C (▪), 40°C (⧫),42°C (●), and 44°C (▴).
