TABLE 1.
B. subtilis and S. pneumoniae strains used in this study
| Strain | Relevant genotype | Source or referencea |
|---|---|---|
| B. subtilis | ||
| QB4991 | trpC2 ΔctsR amyE::(′lacZ aphA3) | 11 |
| QB8068 | trpC2 ΔctsR amyE::(′lacZ cat) | pAC5→QB4991 |
| QB8069 | trpC2 ΔctsR amyE::(′lacZ aphA3) thrC::(PxylA-ctsR spc) | pxyl-ctsR-Spn→QB4991 |
| QB8070 | trpC2 ΔctsR amyE::(clpP′-bgaB cat) thrC::(PxylA-ctsR spc) | pDL-clpP-Spn→QB8069 |
| QB8071 | trpC2 ΔctsR amyE::(clpC′-bgaB cat) thrC::(PxylA-ctsR spc) | pDL-clpC-Spn→QB8069 |
| QB8132 | trpC2 ΔctsR amyE::(groES′-bgaB aphA3) | pDK-groE-Spn→QB8068 |
| QB8133 | trpC2 ΔctsR amyE::(groES′-bgaB aphA3) thrC::(PxylA-ctsR spc) | pxyl-ctsR-Spn→QB8132 |
| QB8134 | trpC2 ΔctsR amyE::(clpE′-bgaB aphA3) | pDK-clpE-Spn→QB8068 |
| QB8135 | trpC2 ΔctsR amyE::(clpE′-bgaB aphA3) thrC::(PxylA-ctsR spc) | pxyl-ctsR-Spn→QB8134 |
| S. pneumoniae | ||
| R6 | Subclone of R36A original isolate | Laboratory stock |
| R800 | R6 derivative | 27 |
| R348 | ebg::spc comC::pXF520 | 29 |
| R354 | ebg::kan comC::pXF520 | 29 |
| R461 | ebg::kan comC::pXF520 spc93::clpP | This study |
| R638 | spc93::clpP | R461→R800 |
| SP2000 | ebg::spc comC::pXF520 ΔclpP::aphA3 | ΔclpP DNA→R348 |
| SP2001 | ebg::spc comC::pXF520 ΔclpC::aphA3 | ΔclpC DNA→R348 |
| SP2002 | ebg::spc comC::pXF520 ΔclpE::aphA3 | ΔclpE DNA→R348 |
| SP2003 | ebg::spc comC::pXF520 ΔctsR::aphA3 | ΔctsR DNA→R348 |
| R895 | ssbB::pR424 | pR424→R800 |
| R1053 | ssbB::pR424 ΔclpE::aphA3 | SP2002→R895 |
| R1054 | ssbB::pR424 spc93::clpP | R461→R895 |
| R1055 | ssbB::pR424 ΔctsR::aphA3 | SP2003→R895 |
| R1056 | ssbB::pR424 ΔclpC::aphA3 | SP2001→R895 |
a
Arrows indicate construction by transformation with chromosomal or plasmid DNA or PCR-generated DNA fragments.