pDG1731 |
Plasmid allowing integration at the thrC locus |
18 |
pXT |
pDG1731 derivative allowing gene expression from the PxylA xylose-inducible promoter |
10 |
pxyl-ctsR-Spn
|
pXT derivative carrying the ctsR coding sequence of S. pneumoniae
|
This study |
pDL |
Plasmid allowing integration at the amyE locus, and transcriptional fusion with bgaB
|
61 |
pAC5 |
Plasmid allowing integration at the amyE locus, with the pC194 cat gene |
30 |
pAC7 |
Plasmid allowing integration at the amyE locus, with the E. faecalis aphA3 cassette |
60 |
pDK |
pAC7 derivative carrying the bgaB gene |
This study |
pDL-clpC-Spn
|
pDL derivative carrying a clpC′-bgaB fusion |
This study |
pDL-clpP-Spn
|
pDL derivative carrying a clpP′-bgaB fusion |
This study |
pDK-clpE-Spn
|
pDK derivative carrying a clpE′-bgaB fusion |
This study |
pDKgroES-Spn
|
pDK derivative carrying a groE′-bgaB fusion |
This study |
pET28a |
Vector for overexpression of His-tagged proteins |
Novagen |
pETCtsR-Spn
|
pET28a derivative for overproduction of CtsR
|
This study |
p5.00 |
Plasmid carrying the luc reporter gene |
Martin Stieger |
pEVP3 |
Plasmid containing the Cmr-encoding gene |
7 |
pR412 |
Plasmid carrying the mariner minitransposon |
29 |
pR422 |
p5.00 derivative with the luc gene under control of the ssbB promoter |
This study |
pR424 |
pEVP3 derivative carrying the ssbB′-luc fusion from pR422, associated with the Cmr-encoding gene |
This study |