TABLE 2.
Plasmids used in this study
| Plasmid | Description | Source or reference |
|---|---|---|
| pDG1731 | Plasmid allowing integration at the thrC locus | 18 |
| pXT | pDG1731 derivative allowing gene expression from the PxylA xylose-inducible promoter | 10 |
| pxyl-ctsR-Spn | pXT derivative carrying the ctsR coding sequence of S. pneumoniae | This study |
| pDL | Plasmid allowing integration at the amyE locus, and transcriptional fusion with bgaB | 61 |
| pAC5 | Plasmid allowing integration at the amyE locus, with the pC194 cat gene | 30 |
| pAC7 | Plasmid allowing integration at the amyE locus, with the E. faecalis aphA3 cassette | 60 |
| pDK | pAC7 derivative carrying the bgaB gene | This study |
| pDL-clpC-Spn | pDL derivative carrying a clpC′-bgaB fusion | This study |
| pDL-clpP-Spn | pDL derivative carrying a clpP′-bgaB fusion | This study |
| pDK-clpE-Spn | pDK derivative carrying a clpE′-bgaB fusion | This study |
| pDKgroES-Spn | pDK derivative carrying a groE′-bgaB fusion | This study |
| pET28a | Vector for overexpression of His-tagged proteins | Novagen |
| pETCtsR-Spn | pET28a derivative for overproduction of CtsR | This study |
| p5.00 | Plasmid carrying the luc reporter gene | Martin Stieger |
| pEVP3 | Plasmid containing the Cmr-encoding gene | 7 |
| pR412 | Plasmid carrying the mariner minitransposon | 29 |
| pR422 | p5.00 derivative with the luc gene under control of the ssbB promoter | This study |
| pR424 | pEVP3 derivative carrying the ssbB′-luc fusion from pR422, associated with the Cmr-encoding gene | This study |