TABLE 3.
Oligonucleotides used in this study
| Name | Sequence | Description |
|---|---|---|
| TM289 | GAAGAATTCGAAAAAGAAGAATGACTTGG | PclpP′-bgaB fusion |
| TM290 | GGAGGATCCTTTTGAGTTTTAATTTTGTTGG | PclpP′-bgaB fusion |
| TM291 | GAAGAATTCCACGCTTGGTATCTTGAGATTCAC | PclpC′-bgaB fusion |
| TM292 | GGAGGATCCTCTCTTTAAACCTTGACCTTG | PclpC′-bgaB fusion |
| AC6 | CTCCTCGAGCTTCCCTTTTCTATCTACCTC | CtsR overproduction |
| AC7 | GGAGGATCCGTTTAAAGAGAGAGGTGGGTTTGT | PxylA′-ctsR fusion |
| AC8 | GAAGAATTCAATAGTTCATCTTACTTCCCT | PxylA′-ctsR fusion |
| AC9 | GGTGGTCTCCCATGAGATTTAAAAATACATCGGATCATA | CtsR overproduction |
| AC22 | TCGAAAAGAAGAATGACTTGG | PclpP footprint |
| AC23 | TCCTTTTGAGTTTTAATTTTGTTGG | PclpP footprint |
| AC24 | GAATTAGGCTTAGATAAGTAG | PclpC footprint |
| AC25 | CCAGATTGATCTAAAATCGCC | PclpC footprint |
| AC72 | CGTAAGAACGTTCTCCACGGCTTGTTTG | Primer extension on clpP |
| AC80 | ATTGAATTCATCGCAATGGAAATTTACGAAC | PclpE′-bgaB fusion |
| AC81 | AAAGGATCCATCTACCTCATTTCTTTCTTTAGCC | PclpE′-bgaB fusion |
| AC82 | GTGAGAATTCTGCAGGCCAAGATTTGGCAG | PgroE′-bgaB fusion |
| AC83 | TCTGGATCCCTCCATAATGAGATAG | PgroE′-bgaB fusion |
| AC84 | GTTAGAATTCGCTTCTTGGGGTAT | aphA3 cassette |
| AC85 | TAGGGATCCAAATCTAGGTACTAA | aphA3 cassette |
| AC86 | TCCCCATGGACTTAGCGGTGGGATG | ctsR deletion |
| AC87 | ATCGAATTCCAAACCCACCTCTCTC | ctsR deletion |
| AC88 | AAGGGATCCAAGATGAACTATTCAAAAGC | ctsR deletion |
| AC89 | GTACCATGGCGTTGGCGTAAAGCC | ctsR deletion |
| AC90 | AAACCATGGAATGAATGTATCGAAAGTGCC | clpC deletion |
| AC91 | GTACGAATTCGTTGGCGTAAAGCC | clpC deletion |
| AC92 | TGGGGATCCGGATATTCGTTTTGACCAGG | clpC deletion |
| AC93 | ATGCCATGGCAAATTTTAACTGGCCTGC | clpC deletion |
| AC94 | TGACCATGGTTCCAGCTGCTAAAGTTGGC | clpP deletion |
| AC95 | TTTGAATTCTAATTTTGTTGGTCAAATG | clpP deletion |
| AC96 | ACTGGATCCGCGCCCAGGAAACACTTG | clpP deletion |
| AC97 | GCTACCATGGCAAGCGCCACAAACGATAG | clpP deletion |
| AC98 | CCTCCATGGTAAAATAGTAACGATAAG | clpE deletion |
| AC99 | CCTGAATTCTTTAAAGGTCAAAAATAG | clpE deletion |
| AC100 | GCAAGGGATCCATTCAGATTAAATCTGCC | clpE deletion |
| AC101 | ATACCATGGATAATGCAAGATTCC | clpE deletion |
| AC112 | TTTTCATTGTAACAACTTCTCAAAGC | PclpE footprint |
| AC113 | CCATTGAGATTGGTGTAAAGATG | PclpE footprint |
| AC114 | GATTTGGCAGATTTGGTCTTGG | PgroE footprint |
| AC115 | CGGTCCCCTAATGGTTTCAAC | PgroE footprint |
| AC124 | AGCCTGCAAGGACAAAGCCTCC | Primer extension on groE |
| MP122 | CGCGGATCCGGTGTAGACGTTAAACGTCC | ssbB′-luc fusion |
| MP158 | GCCGCGAAGCTTCTCAGGATATTGCAGATAC | ssbB′-luc fusion |