| Antibodies |
|
| anti-CD45 |
Biolegend |
103133; RRID: AB_10899570 |
| anti-CD34 |
Biolegend |
128609; RRID: AB_10899570 |
| anti-CD140a |
Biolegend |
135911; RRID: AB_10899570 |
| anti-SCA1 |
Biolegend |
122511; RRID: AB_10899570 |
| anti-SCA1 |
Thermo Fisher Scientific |
710952; RRID: AB_2609699 |
| anti-PDGFRA |
Origene |
TA807645 |
| anti-LAMA4 |
R&D Systems |
AF3837; RRID: AB_2249744 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Isoflurane |
Piramal Healthcare |
60307-120 |
| Gill 3 Hematoxylin |
Richard Allan Scientific |
72604 |
| Eosin Y |
Richard Allan Scientific |
71311 |
| Cytox green |
Invitrogen |
S34860 |
|
| Critical commercial assays |
|
| Direct-zol RNA MiniPrep |
Zymo Research |
R2050 |
| Advantage 2 Polymerase Mix |
TaKaRa Bio |
639201 |
| Adipose tissue dissociation kit |
Miltenyi Biotec |
130-105-808 |
| Skeletal muscle tissue dissociation kit |
Miltenyi Biotec |
130-098-305 |
| Chromium Single Cell 3’ RNA reagent kit v3 |
10X Genomics |
1000075 |
| Dead cell removal MS column |
Miltenyi Biotec |
130-042-201 |
| Dead cell removal kit |
Miltenyi Biotec |
130-090-101 |
| miRNeasy micro kit |
Qiagen |
217084 |
| RNAscope LS Multiplex Reagent Kit |
Advanced Cell Diagnostics |
322800 |
|
| Deposited data |
|
| Mouse scWAT, vWAT and SkM exercise-obesity bulk RNA-seq data |
This paper |
GEO: GSE183239
|
| Mouse scWAT, vWAT and SkM exercise-obesity single-cell RNA-seq data |
This paper |
GEO: GSE183288
|
| Raw data |
This paper |
Data S1 |
|
| Experimental models: Organisms/strains |
|
| C57BL/6 N mice |
Charles River Laboratories |
N/A |
|
| Oligonucleotides |
|
| See bulk mRNA sequencing section for oligos and primers |
N/A |
N/A |
| Pdgfra-F: AGAGTTACACGTTTGAGCTGTC; Pdgfra-R: GTCCCTCCACGGTACTCCT |
Integrated DNA Technologies |
N/A |
| Ly6a-F: AGGAGGCAGCAGTTATTGTGG; Ly6a-R: CGTTGACCTTAGTACCCAGGA |
Integrated DNA Technologies |
N/A |
| Dpt-F: TGGATGGGTGAATCTTAACCGC; Dpt-R: TCAGAGCCTTCCTTCTTGCTA |
Integrated DNA Technologies |
N/A |
| Apoe-F: CTGACAGGATGCCTAGCCG; Apoe-R: CGCAGGTAATCCCAGAAGC |
Integrated DNA Technologies |
N/A |
| Mm-Ly6a-C3 |
Advanced Cell Diagnostics |
427578-C3 |
| Mm-Pdgfra-C4 |
Advanced Cell Diagnostics |
480668-C4 |
|
| Software and algorithms |
|
| CellProfiler 3.0 |
(McQuin et al., 2018) |
http://cellprofiler.org
|
| Salmon 0.14.2 |
(Patro et al., 2017) |
https://salmon.readthedocs.io/en/latest/salmon.html
|
| CIBERSORTx |
(Newman et al., 2019) |
https://cibersortx.stanford.edu/
|
| Cell Ranger software v4.0.0 |
10X Genomics |
https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger
|
| CellPhoneDB |
(Efremova et al., 2020) |
https://github.com/ventolab/CellphoneDB
|
| Cytoscape |
(Shannon et al., 2003) |
https://cytoscape.org/
|
| R 4.0.4 |
See quantification and statistical analysis section for R packages used |
https://www.r-project.org/about.html
|
| STAR v2.5.2b |
(Dobin et al., 2013) |
https://github.com/alexdobin/STAR
|
| Kallisto |
(Bray et al., 2016) |
https://pachterlab.github.io/kallisto/about
|
| FastQTL |
(Ongen et al., 2016) |
https://github.com/francois-a/fastqtl
|
| Metascape |
(Zhou et al., 2019) |
https://metascape.org/gp/index.html#/main/step1
|
| Prism v9 |
GraphPad Software |
http://www.graphpad.com
|
|
| Other |
|
| 10% kcal fat LabDiet |
PharmaServ |
9F5020 |
| 60% kcal fat LabDiet |
PharmaServ |
9F5020 |
| 24 cm running wheel |
NalGene |
N/A |
