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Spiked control IVT signal as a function of concentration. Final concentrations of the four control in vitro transcripts in the 200-μl hybridization cocktail (assuming an equimolar reverse transcription from RNA template to biotin-labeled cDNA): 40 pM Bleor gene, 10 pM GFP gene, 2 pM Specr gene, and 0.5 pM Eryr gene. Plotted lines are for each of the eight microarray samples (two strains; four time points).
