TABLE 3.
Sporulation genes affected by the scoC4 mutation
| Gene | Change (fold)a | Time pointb | Description |
|---|---|---|---|
| bofA | 4.4 | t4 | Inhibition of the pro-sigma-K processing machinery |
| cotE | 3.7 | t4 | Spore coat protein (outer) |
| cotJA | 4.6 | t4 | Polypeptide composition of the spore coat |
| cotJB | 4.1 | t4 | Polypeptide composition of the spore coat |
| cotJC | 4.4 | t4 | Polypeptide composition of the spore coat |
| cotY | 7.8 | t4 | Spore coat protein (insoluble fraction) |
| dacF | 6.1 | t4 | Penicillin-binding protein (putative Ala-Ala carboxypeptidase) |
| phrA | −3.0 | t2 | Phosphatase (RapA) inhibitor |
| rapA | −3.5 | t1 | Response regulator aspartate phosphatase |
| sinI | 2.3 | t2 | Antagonist of SinR |
| spmA | 4.8 | t4 | Spore maturation protein (spore core dehydration) |
| spmB | 4.7 | t4 | Spore maturation protein (spore core dehydration) |
| spoIID | 3.9 | t4 | Required for complete dissolution of the asymmetric septum |
| spoIIIAA | 5.7 | t4 | Mutants block sporulation after engulfment |
| spoIIIAB | 3.6 | t4 | Mutants block sporulation after engulfment |
| spoIIIAC | 3.3 | t4 | Mutants block sporulation after engulfment |
| spoIIIAD | 3.9 | t4 | Mutants block sporulation after engulfment |
| spoIIIAF | 3.5 | t4 | Mutants block sporulation after engulfment |
| spoIIIAG | 3.1 | t4 | Mutants block sporulation after engulfment |
| spoIIIAH | 3.3 | t4 | Mutants block sporulation after engulfment |
| spoIIP | 4.7 | t4 | Required for dissolution of the septal cell wall |
| spoIIQ | 2.9 | t4 | Required for completion of engulfment |
| spoIVA | 4.1 | t4 | Required for proper spore cortex formation and coat assembly |
| spoIVFA | 3.8 | t4 | Inhibition of spoIVFB |
| spoIVFB | 4.3 | t4 | Required for the processing of pro-sigma-K to active sigma-K |
| spoVB | 4.9 | t4 | Involved in spore cortex synthesis |
| spoVID | 6.1 | t4 | Required for assembly of the spore coat |
| spoVK | 4.8 | t4 | Disruption leads to the production of immature spores |
| spoVM | 4.5 | t4 | Required for normal spore cortex and coat synthesis |
| spoVR | 8.4 | t4 | Involved in spore cortex synthesis |
| sspA | 3.5 | t4 | Small acid-soluble spore protein (major alpha-type SASP) |
| sspC | 6.7 | t4 | Small acid-soluble spore protein (minor alpha/beta-type SASP) |
| sspE | 2.9 | t4 | Small acid-soluble spore protein (major gamma-type SASP) |
| tlp | 7.3 | t4 | Small acid-soluble spore protein (thioredoxin-like protein) |
| usd | 8.3 | t4 | Required for translation of spoIIID |
| yknT | 7.1 | t4 | Unknown; sporulation protein sigma-E- controlled |
| ykvU | 5.4 | t4 | Unknown; similar to spore cortex membrane protein |
| yrbA | 3.8 | t4 | Unknown; similar to spore coat protein |
a
A value greater than 0 indicates higher scoC mutant expression relative to wild type.
b
See Fig. 1.