FIGURE 4.

M3 attenuates rmCIRP-induced renal endothelial cell activation. HRGEC were treated with 10 μg/ml of M3 or PBS vehicle for 30 min, then stimulated with 1 μg/ml of rmCIRP. After 20 h, IL-6 protein in the (A) supernatant and (B) intracellularly was measured using ELISA. (C) sTREM-1 in the supernatant was measured using ELISA, n = 6–10 samples per group. (D) Relative secretion of 105 cytokines, chemokines, and growth factors in the supernatant were visualized in duplicate using a cytokine array and (E) pixel densities of upregulated factors were measured, n = 1 sample per group. All groups were compared by one-way ANOVA and Tukey’s method. *p < 0.05 vs. control, ^# p < 0.05 vs. vehicle. Data are expressed as mean ± SEM.