Figure 2.

Structure and intracellular localization of a fluorescent SARS-CoV-2 reporter. (A) Scheme of the fluorescent reporter. EGFP: enhanced GFP (green), NLS: nuclear localization signal (blue), CS: cleavage site (red), cyt5b TM: cytochrome 5b transmembrane domain (yellow). (B) Vero-81-derived clone 6 cells expressing the fluorescent reporter (green) were infected with SARS-CoV-2 (left panels) or left non-infected (right panels). Cells were fixed and processed for immunofluorescent detection of dsRNA (red) and DAPI staining (blue) at 16 hpi. Same fields are shown with combined fluorescent signals (top) or with GFP signal only (bottom). (C) Confocal image of an non-infected clone 6 cell expressing the fluorescent reporter (GFP) immunolabeled with an antibody to calnexin (CNX). Individual channels are shown in grayscales for a better display and combined images in colors in the right panel. Bar, 10 μm.